Aquatic Food Studies 2022, Vol 2, Num, 1     (Pages: AFS57)

Functional Annotation and Characterization of a Hypothetical Protein from Pseudoalteromonas spp. Identify Potential Biomarker: An In-silico Approach

Sk Injamamaul Islam 1 ,Moslema Jahan Mou 2 ,Saloa Sanjida 3 ,Sarower Mahfuj 1

1 Chulalongkorn University, Faculty of Veterinary Science and Technology, Department of Veterinary Microbiology, Bangkok-10330, Thailand
2 University of Rajshahi, Faculty of Life and Earth Science, Department of Genetic Engineering & Biotechnology, Rajshahi-6205, Bangladesh
3 Jashore University of Science and Technology, Faculty of Applied Science and Technology, Department of Environmental Science and Technology, Jashore-7408, Bangladesh
DOI : 10.4194/AFS57 - A wide variety of Pseudoalteromonas spp. found in Kappaphycus alvarezii (marine seaweed) causing deadliest Ice-ice disease and in Laminaria japonica (brown seaweed) found to cause red spots disease. However, very little is known about this pathogen and its genome characteristics. Furthermore, several proteins in its genome are classified as hypothetical proteins (HPs). As a result, the current work sought to elucidate the roles of an HP found in the genome of Pseudoalteromonas spp. To determine the structure and function of this protein, many bioinformatics methods were used. The active site and interacting proteins were examined using CASTp and the STRING server. An important biological activity of the HP is that it contains single functional domains that may be responsible for exopolysaccharide biosynthesis and can be a potential biomarker. Further, protein-protein interactions within selected HP revealed several functional partners that are essential for bacterial survival. In addition, molecular docking and simulation results showed stable bonding between HP and HSP90. Finally, the current work shows that the annotated HP is associated with possible protein sorting signals in the environment as well as having a stable binding with the HSP90, which might be of significant relevance to future bacterial genetics research. Keywords : Hypothetical protein Molecular docking Biomarker Pseudoalteromonas spp